How do I make 2 or more variable never be equal in Python?

First of all, sorry for any vocabulary mistake, I'm brazillian.

I'm new at programming and I've heard that one thing to do to improve your programming skills is try to create some projects, just for fun, and I'm trying to create some kind of Q&A game using the random function to shuffle the alternatives (A, B, C, D, E) at each try. Here is an example of what I want to do.

import random

list = [1, 2, 3, 4, 5]

n1 = (random.choice(list))
n2 = (random.choice(list))
n3 = (random.choice(list))
n4 = (random.choice(list))
n5 = (random.choice(list))

print("Question 1 - What is the result of 2+2?")

print("A){}.".format(n1))
print("B){}.".format(n2))
print("C){}.".format(n3))
print("D){}.".format(n4))
print("E){}.".format(n5))

The output is:

Question 1 - What is the result of 2+2?

A)4.
B)1.
C)2.
D)2.
E)4.

Process finished with exit code 0

I know its barely impossible to make each alternative be different every time with this code, and my question is: How can i make sure the alternatives will never be equal?