Python: Choose random line from file, then delete that line

I'm new to Python (in that I learned it through a CodeAcademy course) and could use some help with figuring this out.

I have a file, 'TestingDeleteLines.txt', that's about 300 lines of text. Right now, I'm trying to get it to hand me 10 random lines from that file, then delete those lines.

The issue is, when Python reads a file, it reads that file and once it gets to the end, it won't go back and delete the lines. My current thinking was that I could write the lines to a list, then reopen the file, match the list to the text file, and if it finds a match, delete the lines.

My current problem is twofold:

1. It's duplicating the random elements. If it picks a line, I need it to not pick that same line again. However, using random.sample doesn't seem to work, as I need those lines separated out when I later use each line to append to a URL.

2. I don't feel like my logic (write to array->find matches in text file->delete) is the most ideal logic. Is there a better way to write this?

   import webbrowser
   import random
   
   """url= 'http://www.google.com'
   webbrowser.open_new_tab(url+myline)""" Eventually, I need a base URL + my 10 random lines opening in each new tab
   
   def ShowMeTheRandoms():
       x=1
       DeleteList= []
       lines=open('TestingDeleteLines.txt').read().splitlines()
   for x in range(0,10):
       myline=random.choice(lines)
       print(myline) """debugging, remove later"""
       DeleteList.append(myline)
       x=x+1
       print DeleteList """debugging, remove later"""
   ShowMeTheRandoms()